背景描述: 該系由A.F. Gazdar,H.K. Oie,J.D. Minna及其同事于1981年從患者開始化療前獲得的腫瘤組織中得出。超微結構研究表明,存在 Clara 細胞特征的細胞質結構。
培養(yǎng)建議
生長形態(tài): 上皮細胞樣
生長特性: 貼壁
培養(yǎng)條件: RPMI-1640+10%FBS+1%P/S
凍存條件: 90%FBS+10%DMSO
血清使用等級: 標準
傳代方式: 胰酶消化
倍增時間: 38~60h
傳代比例: 胰酶消化
換液頻率: 每周2~3次
生物安全等級: 1
培養(yǎng)注意事項:
其他信息
基因表達: lung surfactant associated protein A (SP-A); protein and RNA of SP-A, the major lung surfactant associated protein
致瘤性: 裸鼠
核型: modal numbr = 57; range = 53 to 65.This is a hypotriploid human cell line. The modal chromosome number is 57 although cells with 58 chromosomes occurred with a comparable frequency. The frequency of higher ploidies was 1.7%. Seven marker chromosomes, der(9)t(1;9)(q21;p24), der(9)t(7;9)(p11;p22), t(10q14q), der(16)t(7;16)(q11.23;q22), a small ring (about 1/2 the size of a G chromosome) and two others, were common to all cells.
人非小細胞肺癌細胞
Catalog NoCL0236
Product information
基因表達: lung surfactant associated protein A (SP-A); protein and RNA of SP-A, the major lung surfactant associated protein
致瘤性: 裸鼠
核型: modal numbr = 57; range = 53 to 65.This is a hypotriploid human cell line. The modal chromosome number is 57 although cells with 58 chromosomes occurred with a comparable frequency. The frequency of higher ploidies was 1.7%. Seven marker chromosomes, der(9)t(1;9)(q21;p24), der(9)t(7;9)(p11;p22), t(10q14q), der(16)t(7;16)(q11.23;q22), a small ring (about 1/2 the size of a G chromosome) and two others, were common to all cells.