背景描述: T98G [T98-G] 是一種成纖維細(xì)胞樣細(xì)胞,是從多形性膠質(zhì)母細(xì)胞瘤 White 的大腦中分離出來(lái)的
培養(yǎng)建議
生長(zhǎng)形態(tài): 成纖維細(xì)胞樣
生長(zhǎng)特性: 貼壁
培養(yǎng)條件: MEM+10%FBS+1%P/S
凍存條件: 90%FBS+10%DMSO
血清使用等級(jí): 標(biāo)準(zhǔn)
傳代方式: 胰酶消化
倍增時(shí)間: 28~40h
傳代比例: 胰酶消化
換液頻率: 每周2~3次
生物安全等級(jí): 1
培養(yǎng)注意事項(xiàng):
其他信息
核型: This is a human cell line with hyperpentaploid chromosome count. The modal chromosome number should be around 128 to 132. The rate of cells with higher ploidies was 1.39%. Fourteen to 16 marker chromosomes were common to most cells. They were: der(1)t(1;?) (p36;?), i(6p), der(10)t(10;?) (q24;?), der (19)t(19;?) (q13;?), der(15)t(15;?) (q26?;?), minute metacentric and eight to ten others. Most of these structurally altered markers had complex interchromosomal exchanges. The der(10) and der(19) could be formed from a balanced translocation, i.e., t(10;19) (q24;q13). These two markers and the minute metacentric were present in three or more copies in most cells. There were six or more copies for N5, N7, N11, N13, N20, N21, and N22 in most cells. The X and N15 had only one copy.
人膠質(zhì)母細(xì)胞瘤
Catalog NoCL0311
Product information
核型: This is a human cell line with hyperpentaploid chromosome count. The modal chromosome number should be around 128 to 132. The rate of cells with higher ploidies was 1.39%. Fourteen to 16 marker chromosomes were common to most cells. They were: der(1)t(1;?) (p36;?), i(6p), der(10)t(10;?) (q24;?), der (19)t(19;?) (q13;?), der(15)t(15;?) (q26?;?), minute metacentric and eight to ten others. Most of these structurally altered markers had complex interchromosomal exchanges. The der(10) and der(19) could be formed from a balanced translocation, i.e., t(10;19) (q24;q13). These two markers and the minute metacentric were present in three or more copies in most cells. There were six or more copies for N5, N7, N11, N13, N20, N21, and N22 in most cells. The X and N15 had only one copy.